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combination with acv  (MedChemExpress)


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    MedChemExpress combination with acv
    Effect of <t>berberine</t> <t>(BBR)</t> combined with acyclovir <t>(ACV)</t> on GM130 on TLR3 and inflammatory cytokines. a Cellular activity of cells treated with different concentrations of BBR for 24 h. Selection of BBR at a concentration of 3 μM pretreated for 12 h followed by HSV-1 infection for 12 h, and collection of samples. Cells are pretreated with ACV (3 μM) for 12 h and infected with HSV-1 for 12 h. b Protein levels of GM130, TLR3, and HSV-1 treated with BBR and ACV were adjusted with βactin as the internal reference. The expression levels of the detected proteins in each group were compared with those of the uninfected group. c Semi-quantitative results of GM130. d Semi-quantitative results of TLR3. e Semi-quantitative results of HSV-1-gD. f – h Secretion levels of the inflammatory cytokines IFN-β, TNF-α, and IL-6 after treatment with BBR and ACV. i Viral titers are treated with BBR and ACV. Data are obtained through three independent replicates, expressed as X ± SD, and statistically analyzed between the two groups using a t-test. A comparison between the two groups is marked with a dashed line. ns, p > 0.05; *, #, p < 0.05; **, ##, p < 0.01; ***, ###, p < 0.001
    Combination With Acv, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "HSV-1 immune escapes in microglia by down-regulating GM130 to inhibit TLR3-mediated innate immune responses"

    Article Title: HSV-1 immune escapes in microglia by down-regulating GM130 to inhibit TLR3-mediated innate immune responses

    Journal: Virology Journal

    doi: 10.1186/s12985-024-02492-x

    Effect of berberine (BBR) combined with acyclovir (ACV) on GM130 on TLR3 and inflammatory cytokines. a Cellular activity of cells treated with different concentrations of BBR for 24 h. Selection of BBR at a concentration of 3 μM pretreated for 12 h followed by HSV-1 infection for 12 h, and collection of samples. Cells are pretreated with ACV (3 μM) for 12 h and infected with HSV-1 for 12 h. b Protein levels of GM130, TLR3, and HSV-1 treated with BBR and ACV were adjusted with βactin as the internal reference. The expression levels of the detected proteins in each group were compared with those of the uninfected group. c Semi-quantitative results of GM130. d Semi-quantitative results of TLR3. e Semi-quantitative results of HSV-1-gD. f – h Secretion levels of the inflammatory cytokines IFN-β, TNF-α, and IL-6 after treatment with BBR and ACV. i Viral titers are treated with BBR and ACV. Data are obtained through three independent replicates, expressed as X ± SD, and statistically analyzed between the two groups using a t-test. A comparison between the two groups is marked with a dashed line. ns, p > 0.05; *, #, p < 0.05; **, ##, p < 0.01; ***, ###, p < 0.001
    Figure Legend Snippet: Effect of berberine (BBR) combined with acyclovir (ACV) on GM130 on TLR3 and inflammatory cytokines. a Cellular activity of cells treated with different concentrations of BBR for 24 h. Selection of BBR at a concentration of 3 μM pretreated for 12 h followed by HSV-1 infection for 12 h, and collection of samples. Cells are pretreated with ACV (3 μM) for 12 h and infected with HSV-1 for 12 h. b Protein levels of GM130, TLR3, and HSV-1 treated with BBR and ACV were adjusted with βactin as the internal reference. The expression levels of the detected proteins in each group were compared with those of the uninfected group. c Semi-quantitative results of GM130. d Semi-quantitative results of TLR3. e Semi-quantitative results of HSV-1-gD. f – h Secretion levels of the inflammatory cytokines IFN-β, TNF-α, and IL-6 after treatment with BBR and ACV. i Viral titers are treated with BBR and ACV. Data are obtained through three independent replicates, expressed as X ± SD, and statistically analyzed between the two groups using a t-test. A comparison between the two groups is marked with a dashed line. ns, p > 0.05; *, #, p < 0.05; **, ##, p < 0.01; ***, ###, p < 0.001

    Techniques Used: Activity Assay, Selection, Concentration Assay, Infection, Expressing, Comparison

    Structural changes of the Golgi apparatus (GA) in microglia after treatment with acyclovir (ACV) and berberine (BBR). ACV and BBR were pretreated for 12 h before infection, HSV-1 infected cells with MOI = 1, and GA structure (GM130 marker, P115 marker) is observed 12 h after infection. Scale, 10 μm. After ACV treatment, the fluorescently labeled GA structure of GM130 and P115 partially recovered compact perinuclear distribution. After BBR treatment, the fluorescently labeled GA structure of GM130 and P115 partially recovered compact perinuclear distribution. After ACV is combined with BBR, the fluorescently labeled GA structure of GM130 and P115 is further restored to a compact perinuclear distribution. The representative images are obtained from three independent repeated experiments. Scale, 10 μm
    Figure Legend Snippet: Structural changes of the Golgi apparatus (GA) in microglia after treatment with acyclovir (ACV) and berberine (BBR). ACV and BBR were pretreated for 12 h before infection, HSV-1 infected cells with MOI = 1, and GA structure (GM130 marker, P115 marker) is observed 12 h after infection. Scale, 10 μm. After ACV treatment, the fluorescently labeled GA structure of GM130 and P115 partially recovered compact perinuclear distribution. After BBR treatment, the fluorescently labeled GA structure of GM130 and P115 partially recovered compact perinuclear distribution. After ACV is combined with BBR, the fluorescently labeled GA structure of GM130 and P115 is further restored to a compact perinuclear distribution. The representative images are obtained from three independent repeated experiments. Scale, 10 μm

    Techniques Used: Infection, Marker, Labeling



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    Effect of <t>berberine</t> <t>(BBR)</t> combined with acyclovir <t>(ACV)</t> on GM130 on TLR3 and inflammatory cytokines. a Cellular activity of cells treated with different concentrations of BBR for 24 h. Selection of BBR at a concentration of 3 μM pretreated for 12 h followed by HSV-1 infection for 12 h, and collection of samples. Cells are pretreated with ACV (3 μM) for 12 h and infected with HSV-1 for 12 h. b Protein levels of GM130, TLR3, and HSV-1 treated with BBR and ACV were adjusted with βactin as the internal reference. The expression levels of the detected proteins in each group were compared with those of the uninfected group. c Semi-quantitative results of GM130. d Semi-quantitative results of TLR3. e Semi-quantitative results of HSV-1-gD. f – h Secretion levels of the inflammatory cytokines IFN-β, TNF-α, and IL-6 after treatment with BBR and ACV. i Viral titers are treated with BBR and ACV. Data are obtained through three independent replicates, expressed as X ± SD, and statistically analyzed between the two groups using a t-test. A comparison between the two groups is marked with a dashed line. ns, p > 0.05; *, #, p < 0.05; **, ##, p < 0.01; ***, ###, p < 0.001
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    Image Search Results


    Effect of berberine (BBR) combined with acyclovir (ACV) on GM130 on TLR3 and inflammatory cytokines. a Cellular activity of cells treated with different concentrations of BBR for 24 h. Selection of BBR at a concentration of 3 μM pretreated for 12 h followed by HSV-1 infection for 12 h, and collection of samples. Cells are pretreated with ACV (3 μM) for 12 h and infected with HSV-1 for 12 h. b Protein levels of GM130, TLR3, and HSV-1 treated with BBR and ACV were adjusted with βactin as the internal reference. The expression levels of the detected proteins in each group were compared with those of the uninfected group. c Semi-quantitative results of GM130. d Semi-quantitative results of TLR3. e Semi-quantitative results of HSV-1-gD. f – h Secretion levels of the inflammatory cytokines IFN-β, TNF-α, and IL-6 after treatment with BBR and ACV. i Viral titers are treated with BBR and ACV. Data are obtained through three independent replicates, expressed as X ± SD, and statistically analyzed between the two groups using a t-test. A comparison between the two groups is marked with a dashed line. ns, p > 0.05; *, #, p < 0.05; **, ##, p < 0.01; ***, ###, p < 0.001

    Journal: Virology Journal

    Article Title: HSV-1 immune escapes in microglia by down-regulating GM130 to inhibit TLR3-mediated innate immune responses

    doi: 10.1186/s12985-024-02492-x

    Figure Lengend Snippet: Effect of berberine (BBR) combined with acyclovir (ACV) on GM130 on TLR3 and inflammatory cytokines. a Cellular activity of cells treated with different concentrations of BBR for 24 h. Selection of BBR at a concentration of 3 μM pretreated for 12 h followed by HSV-1 infection for 12 h, and collection of samples. Cells are pretreated with ACV (3 μM) for 12 h and infected with HSV-1 for 12 h. b Protein levels of GM130, TLR3, and HSV-1 treated with BBR and ACV were adjusted with βactin as the internal reference. The expression levels of the detected proteins in each group were compared with those of the uninfected group. c Semi-quantitative results of GM130. d Semi-quantitative results of TLR3. e Semi-quantitative results of HSV-1-gD. f – h Secretion levels of the inflammatory cytokines IFN-β, TNF-α, and IL-6 after treatment with BBR and ACV. i Viral titers are treated with BBR and ACV. Data are obtained through three independent replicates, expressed as X ± SD, and statistically analyzed between the two groups using a t-test. A comparison between the two groups is marked with a dashed line. ns, p > 0.05; *, #, p < 0.05; **, ##, p < 0.01; ***, ###, p < 0.001

    Article Snippet: To observe the effects of BBR hydrochloride (Sigma, Cat#BP1108) and BBR in combination with ACV (MCE, Cat#HY-17422), we used BBR (0, 3, 10 uM), ACV (3 μM), and BBR (3 uM) + ACV (3 uM) treating BV2 cells separately at 24 h before sample collection.

    Techniques: Activity Assay, Selection, Concentration Assay, Infection, Expressing, Comparison

    Structural changes of the Golgi apparatus (GA) in microglia after treatment with acyclovir (ACV) and berberine (BBR). ACV and BBR were pretreated for 12 h before infection, HSV-1 infected cells with MOI = 1, and GA structure (GM130 marker, P115 marker) is observed 12 h after infection. Scale, 10 μm. After ACV treatment, the fluorescently labeled GA structure of GM130 and P115 partially recovered compact perinuclear distribution. After BBR treatment, the fluorescently labeled GA structure of GM130 and P115 partially recovered compact perinuclear distribution. After ACV is combined with BBR, the fluorescently labeled GA structure of GM130 and P115 is further restored to a compact perinuclear distribution. The representative images are obtained from three independent repeated experiments. Scale, 10 μm

    Journal: Virology Journal

    Article Title: HSV-1 immune escapes in microglia by down-regulating GM130 to inhibit TLR3-mediated innate immune responses

    doi: 10.1186/s12985-024-02492-x

    Figure Lengend Snippet: Structural changes of the Golgi apparatus (GA) in microglia after treatment with acyclovir (ACV) and berberine (BBR). ACV and BBR were pretreated for 12 h before infection, HSV-1 infected cells with MOI = 1, and GA structure (GM130 marker, P115 marker) is observed 12 h after infection. Scale, 10 μm. After ACV treatment, the fluorescently labeled GA structure of GM130 and P115 partially recovered compact perinuclear distribution. After BBR treatment, the fluorescently labeled GA structure of GM130 and P115 partially recovered compact perinuclear distribution. After ACV is combined with BBR, the fluorescently labeled GA structure of GM130 and P115 is further restored to a compact perinuclear distribution. The representative images are obtained from three independent repeated experiments. Scale, 10 μm

    Article Snippet: To observe the effects of BBR hydrochloride (Sigma, Cat#BP1108) and BBR in combination with ACV (MCE, Cat#HY-17422), we used BBR (0, 3, 10 uM), ACV (3 μM), and BBR (3 uM) + ACV (3 uM) treating BV2 cells separately at 24 h before sample collection.

    Techniques: Infection, Marker, Labeling